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Islet of Langerhans basal membrane protein immunostaining. ( A , C , E , G ): Immunohistochemistry of pan-laminin, laminin <t>alpha</t> 1 and gamma 1 chains and alpha 3 integrin. Islet of Langerhans—dashed line. Brown staining indicates protein-of-interest staining. Scale bar: 20.0 µm. ( B , D , F , H ): Quantification of stained area related to total islet area for each analyzed protein. Data presented as mean ± standard error of the mean, * p < 0,02 and *** p < 0.0001 vs. control group by Student’s t -test.
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Islet of Langerhans basal membrane protein immunostaining. ( A , C , E , G ): Immunohistochemistry of pan-laminin, laminin <t>alpha</t> 1 and gamma 1 chains and alpha 3 integrin. Islet of Langerhans—dashed line. Brown staining indicates protein-of-interest staining. Scale bar: 20.0 µm. ( B , D , F , H ): Quantification of stained area related to total islet area for each analyzed protein. Data presented as mean ± standard error of the mean, * p < 0,02 and *** p < 0.0001 vs. control group by Student’s t -test.
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Islet of Langerhans basal membrane protein immunostaining. ( A , C , E , G ): Immunohistochemistry of pan-laminin, laminin <t>alpha</t> 1 and gamma 1 chains and alpha 3 integrin. Islet of Langerhans—dashed line. Brown staining indicates protein-of-interest staining. Scale bar: 20.0 µm. ( B , D , F , H ): Quantification of stained area related to total islet area for each analyzed protein. Data presented as mean ± standard error of the mean, * p < 0,02 and *** p < 0.0001 vs. control group by Student’s t -test.
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Islet of Langerhans basal membrane protein immunostaining. ( A , C , E , G ): Immunohistochemistry of pan-laminin, laminin <t>alpha</t> 1 and gamma 1 chains and alpha 3 integrin. Islet of Langerhans—dashed line. Brown staining indicates protein-of-interest staining. Scale bar: 20.0 µm. ( B , D , F , H ): Quantification of stained area related to total islet area for each analyzed protein. Data presented as mean ± standard error of the mean, * p < 0,02 and *** p < 0.0001 vs. control group by Student’s t -test.
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Islet of Langerhans basal membrane protein immunostaining. ( A , C , E , G ): Immunohistochemistry of pan-laminin, laminin <t>alpha</t> 1 and gamma 1 chains and alpha 3 integrin. Islet of Langerhans—dashed line. Brown staining indicates protein-of-interest staining. Scale bar: 20.0 µm. ( B , D , F , H ): Quantification of stained area related to total islet area for each analyzed protein. Data presented as mean ± standard error of the mean, * p < 0,02 and *** p < 0.0001 vs. control group by Student’s t -test.
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Islet of Langerhans basal membrane protein immunostaining. ( A , C , E , G ): Immunohistochemistry of pan-laminin, laminin alpha 1 and gamma 1 chains and alpha 3 integrin. Islet of Langerhans—dashed line. Brown staining indicates protein-of-interest staining. Scale bar: 20.0 µm. ( B , D , F , H ): Quantification of stained area related to total islet area for each analyzed protein. Data presented as mean ± standard error of the mean, * p < 0,02 and *** p < 0.0001 vs. control group by Student’s t -test.

Journal: Biology

Article Title: Effect of Maternal Hyperglycemia on Fetal Pancreatic Islet Development

doi: 10.3390/biology14060728

Figure Lengend Snippet: Islet of Langerhans basal membrane protein immunostaining. ( A , C , E , G ): Immunohistochemistry of pan-laminin, laminin alpha 1 and gamma 1 chains and alpha 3 integrin. Islet of Langerhans—dashed line. Brown staining indicates protein-of-interest staining. Scale bar: 20.0 µm. ( B , D , F , H ): Quantification of stained area related to total islet area for each analyzed protein. Data presented as mean ± standard error of the mean, * p < 0,02 and *** p < 0.0001 vs. control group by Student’s t -test.

Article Snippet: For protein detection, sections were incubated with specific primary rabbit antibodies: pan-laminin (ab1575; Abcam, diluted 1:500, Cambridge, UK), alpha 1 laminin (sc H-300 Santa Cruz H-300: SC-5582 diluted 1:50, Santa Cruz, CA, USA), gamma 1 laminin (sc5584; Santa Cruz, diluted 1:50), alpha 3 integrin (600-401 R13, Rockland diluted 1:200, Baltimore, MD, USA), anti-PCNA (ab2426 Abcam 1: 200), anti-glucagon (# A0565 1:350) and anti-insulin (# A0564 1:250).

Techniques: Membrane, Immunostaining, Immunohistochemistry, Staining, Control